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產品詳情
  • 產品名稱:細胞增殖檢測

  • 產品型號:
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  • 產品文檔:
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簡單介紹:
細胞增殖是生物體的重要生命特征,細胞以分裂的方式進行增殖。單細胞生物,以細胞分裂的方式產生新的個體。多細胞生物,以細胞分裂的方式產生新的細胞,用來補充體內衰老和死亡的細胞;同時,多細胞生物可以由一個受精卵,經過細胞的分裂和分化,終發育成一個新的多細胞個體。
詳情介紹:

一、達為科優勢

酚紅和血清對CCK8法的檢測不會造成干擾;

WST-8的細胞毒性非常低,因此可以在不同時間反復用酶標儀讀數從而找到佳測定時間。


二、原理

CCK-8檢測細胞增殖(活力):CCK-8(cell-counting kit-8)檢測試劑盒是應用WST-8取代MTT被還原,WST-8是一種類似于MTT的化合物,在電子耦合試劑存在的情況下,可以被線粒體內的一些脫氫酶還原生成橙黃色的formazan。WST-8產生的formazan比XTT和MTS產生的formazan更易溶解。且WST-8相較XTT和MTS化學性狀更穩定, 因此實驗結果相對更加穩定。此外,WST-8相較MTT、XTT,線性范圍相對寬,靈敏度更高。當細胞增殖得越多越快,則formazan產生量越多,顏色越深;反之,當內外因造成的細胞毒性越大,則顏色越淺。對于同樣的細胞,顏色的深淺和細胞數目呈線性關系。應用酶標儀測定吸光值并進行統計學計算便可以獲得細胞增殖(活性)相關數據。


三、服務流程

  1. 制備細胞懸液:細胞計數

  2. 接種到96孔板中,同樣的樣本可做3個重復。

  3. 37℃培養箱中培養。

  4. 加入10ul CCK8

  5. 培養1-4小時

  6. 測定450nm吸光度



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